Figure 4
Increased cortisol converted by 11β-HSD1 inhibits keratinocyte differentiation in vitro (n = 3). (A) mRNA expression of keratinocyte differentiation markers (KRT10, KRT1, and LOR) was decreased by cortisol treatment, but was not affected or only slightly affected by cortisone. (B) Protein expression of KRT10 and KRT1 was decreased by cortisol, but the protein expression of LOR did not differ upon cortisol treatment. Protein expression of KRT10 and LOR was not affected by cortisone. Cortisone-induced protein expression of KRT1 decreased, but the change was less than that observed upon cortisol treatment at the same concentration. Cortisone decreased the mRNA (C) and protein (D) expression of keratinocyte differentiation markers (KRT10 and KRT1) in UVB-exposed keratinocytes. Cortisone decreased the mRNA expression of LOR but did not alter its protein expression in UVB-exposed keratinocytes. *p < 0.05; **p < 0.01; ***p < 0.001.
