Figure 5
From: Acetylcholine and necroptosis are players in follicular development in primates
Immunohistochemical detection of necroptosis-related proteins in macaque ovarian follicles. Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) and 3 (RIPK3) were identified in in vivo-developed preantral (1), small antral (2) and large antral (3) follicles, mainly in granulosa cells (GC) (A). Granulosa cells of some follicles were stained for phosphorylated mixed lineage kinase domain-like protein, pMLKL(S358) (A). Positive staining of pMLKL(S358) was also detected in the granulosa cells of in vitro-developed small antral follicles cultured under control conditions (B; arrows), but not in the negative controls (A,C) or follicles cultured with necrostatin-1 (D). Counterstaining was performed using haematoxylin.
