Figure 3
From: The CPT1a inhibitor, etomoxir induces severe oxidative stress at commonly used concentrations
The specificity of ETO for CPT1a is lost at concentrations above 5 μM T cells expressing either control or shRNA against CPT1A were restimulated with dynabeads and expanded for 5 days. Oxygen consumption rates (OCR) in control (A) versus shRNA cells (C) under basal conditions and after the introduction of ETO (dotted lines) are shown. The corresponding glycolytic rates (ECAR) in control (B) and shRNA cells (D) cells are shown. Representative metabolic parameters from 2 independent experiments are shown. Untransduced T cells were expanded for 8 days with dynabeads and then switched to regular Seahorse assay medium (Medium) or assay medium supplemented with 1 mM sodium acetate (Acetate). Glycolytic rates were measured under basal conditions and following the introduction of 5 μM ETO (E) and 50 μM ETO (F) at the indicated time-points (dotted lines). Representative metabolic parameters from 2 independent experiments are shown.
