Figure 4
From: Targeted mutagenesis in wheat microspores using CRISPR/Cas9
CRISPR/Cas9-based editing of the exogenous DsRed reporter gene in wheat microspores. (A)Fluorescence emitted from DsRed produced in microspores co-transfected with the DsRed_Cas9 and gDsRed-2 constructs (right panel) compared to control cells expressing only DsRed_Cas9 construct (Left panel). (B)Mutations detected at or near the cleavage site in sequenced products of DsRed from microspores co-transfected with the DsRed_Cas9 and gDsRed-2 constructs.
