Figure 5
From: Targeted mutagenesis in wheat microspores using CRISPR/Cas9
CRISPR/Cas9-based editing of the endogenous TaLox2 gene in wheat microspores. (A) Schematic diagram showing the pJIT163-2NLSCas9/gTaLox2-target3 construct used for mutagenesis of TaLox2 gene in wheat microspores. (B) PCR/restriction enzyme assay to detect mutations in the TaLox2 gene induced by Cas9/ gTaLox2. (C) A 2 bp deletion detected at the cleavage site in the sequenced product of TaLox2 from microspores transfected with pJIT163-2NLSCas9/gTaLox2-target3 construct. Sanger sequencing electropherograms are shown below.
