Figure 3
From: CsrA and its regulators control the time-point of ColicinE2 release in Escherichia coli

Accumulation of ssDNA in CWT. Agarose gel of plasmid and ssDNAs extracted from CWT and SREP1 strains. Lanes 1–4 and 9–12 were loaded with the indicated markers: 1-kb ladder, super-coiled ladder; 7249-bp ssDNA ring (M13mp18), and 5386-bp ssDNA ring (PhiX174). Lane 5: uncleaved CWT DNA showing the 6800-bp pColE2-P9 dsDNA (blue) and ssDNA (red). Lane 6: CWT DNA cleaved with PvuI, showing the linearized ds pColE2-P9 plasmid (yellow). Lane 7: uncleaved SREP1 strain DNA showing the 5600-bp reporter plasmid (blue). Lane 8: SREP1 strain DNA cut with PvuI, showing the linearized reporter plasmid (yellow).