Figure 9
From: Amino Acid Signature in Human Melanoma Cell Lines from Different Disease Stages
Alanine and glutamic acid significantly stimulate invasion in slow growing (B), but not in metastatic melanoma cells (A). Human melanoma cells (3,5*104/well) were kept in collagen I coated inner Boyden chambers for 24 hours. The medium in the outer chamber (CTL) was supplemented by L-alanine (100 µg/ml; Ala), L-glutamic acid (200 µg/ml; Glu), L-alanine plus glutamic acid (Ala + Glu) or their combinations with Simvastatin (10 µM; Sim). Data were adjusted for proliferation and normalized to the corresponding time points of the untreated controls. Bars and errors depict the mean ± SEM (n = 4). Significance vs. control treatment was performed with one-way ANOVA and post hoc Student-Newman-Keuls test and indicated with asterisks (*p < 0.05, **p < 0.01, ***p < 0.005). Significance vs. L-alanine or L-glutamic acid were indicated with # or §, respectively (#p < 0.05, ##p < 0.01, §p < 0.05; n.s. denotes not significant).
