Figure 2

The expression of renal lineage markers in CD9⁻CD140a⁺CD140b⁺CD271⁺ cells isolated from hiPSC differentiation culture. (A) Anti-GFP (OSR1), SIX2 and HOXD11 immunostaining images of the cells before isolation (upper panels) and isolated CD9⁻CD140a⁺CD140b⁺CD271⁺ cells (lower panels) on culture days 28 (SIX2) and 30 (OSR1 and HOXD11). Representative images from three independent experiments are shown. Scale bars, 100 µm. (B) The induction rates of SIX2⁺ cells in day 28 cells before and after isolation. The data from three randomly chosen fields are presented as the mean ± SE (n = 3). (C,D) qRT-PCR analyses of gene expressions in day 28 cells before and after isolation for markers of nephron progenitors (C) and undifferentiated cells (D). The data from three independent experiments are presented as the mean ± SE (n = 3). GAPDH was used as an internal control, and the relative expression levels were normalized to those of day 28 cells before isolation (C) and hiPSCs (D). ITGA8, integrin alpha 8; CDH11, cadherin 11.