Figure 2

CyaA-LamB reporter is not translocated by the Dot/Icm T4SS. (a) Adenylate cyclase fusions of LamB expressed in L. pneumophila and infected into hMDMs for 1 hr, in triplicate, using known T4SS effector, RalF, as a positive control. Production of cAMP was assessed by ELISA. Data is shown as mean cAMP concentration ± SD, n = 3 independent infections.