Figure 5

Isoflavone extract inhibits TNF-α-, IL-17A-, and IL-22-induced MAPK and JAK/STAT pathway activation in human primary keratinocytes. Primary keratinocytes were pretreated with different doses of isoflavone extract for 24 hours and then treated with TNF-α, IL-17A, or IL-22. After treatment of TNF-α (A), IL-17A (C), or IL-22 (E), with the quantification data shown in the right panel (B,D,F), the total protein was extracted from the cells and associated protein expression were determined via western blotting. Data are the mean ± SEM from at least three independent experiments. *P < 0.05, indicating significant effects of isoflavone extract as compared to control.