Figure 3 | Scientific Reports

Figure 3

From: Overactive autophagy is a pathological mechanism underlying premature suture ossification in nonsyndromic craniosynostosis

Figure 3

Effect of the modulation of autophagy on cranial SMC mineralization. (A) Representative Alizarin Red S2 staining of mineralization nodules. Samples were cultured in osteogenic medium for 14 days before staining. Insets in each panel are magnified views. (B) Quantification of mineralization. To normalize individual differences, the relative mineralization ratio was calculated for comparison. Details are described in the Materials and Methods section. Data are expressed as the mean ± SD of three independent experiments. *P < 0.05.

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