Figure 1 | Scientific Reports

Figure 1

From: 3D bio-printed scaffold-free nerve constructs with human gingiva-derived mesenchymal stem cells promote rat facial nerve regeneration

Figure 1

Generation of 3D-spheroid GMSCs. (A) 4 × 104 of GMSCs/well in 200 µl of complete stemgro culture medium was seeded into each well of ultra-low attachment round-shaped 96-U well plates and cultured for 48 h. H & E staining of cryosections of GMSC spheroids. Scale bar: 50 µm. (B) Immunocytochemistry showed the expression of a panel of MSC-associated markers CD29, CD73 and CD90 and extracellular components such as type I collagen (col-I), vimentin, fibronectin, and laminin in GMSC-derived spheroids. (C) GMSC spheroids were dissociated into single cells and stained with Annexin V-FITC and 7-AAD to detect early apoptosis and late apoptotic/necrotic cells by flow cytometric analysis. (D) Immunocytochemistry showed that less than 10% of cells inside GMSC spheroids were positive for the cleaved caspase-3. Cell nuclei were counter-stained by DAPI (blue). Scale bar: 50 µm. Data are representative of 3 independent experiments.

Back to article page