Figure 6 | Scientific Reports

Figure 6

From: 3D bio-printed scaffold-free nerve constructs with human gingiva-derived mesenchymal stem cells promote rat facial nerve regeneration

Figure 6

Histological analysis of newly regenerated rat facial nerves. (A) Left panels, H & E staining of cryosections of regenerated facial nerves; Immunohistochemistry showed increased expression of β-tubulin III and organized axonal alignment in regenerated nerves from 3D bio-printed construct transplantation as compared with silicon tube transplantation. (B) Immunohistochemistry showed increased expression of S-100β in regenerated facial nerves from the group with 3D bio-printed construct transplantation as compared with silicon tube control group. Scale bar: 200 µm. Cell nuclei were counter-stained by DAPI (blue). (C) Immunostaining of matured axonal and myelin markers, SMI31/32 and Fluoromyelin, with cryosections of regenerated facial nerves from rats transplanted with 3D bio-printed nerve grafts.

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