Figure 1

Genomic architecture along with relative loci around IFIT genes in human, mouse, dog, horse and chicken, and gene annotation in chicken. (A) The IFIT locus in compared species is flanked upstream with LIPA gene and downstream with PANK1 gene. Direct syntenic analysis identified a single IFIT5 gene in chicken compared to four in other species aslong with other pseudogenes. (B) Transcriptomics profiling of chicken primary fibroblasts which were mock-infected or infected with RB1B strain of MDV. Blue bar represents the transcript in the current chicken genome assembly whereas the red bar represents the mappability of the transcript to the chicken genome. A final transcript from MDV-infected data and gene characterization is shown at the bottom. (C) Phylogenetic analysis of IFIT genes in different species. Based on the clustering patterns and sequence homologies, the single identified gene clustered closer to IFIT5 of duck and human. (D) Putative tetratricopeptide repeats (TPR) showing characteristic features of IFIT proteins. (E) Expression and subcellular distribution of chIFIT5 in chicken embryo fibroblasts. Chicken cells were transfected with 500 ng of mammalian expression vectors encoding V5-tagged chIFIT5 for 24 hours and were left untreated (NDV-) or were treated with 1 MOI of NDV-GFP (NDV+) for another 24 hours before fixation, staining for nucleus (blue), chIFIT5 (red) and GFP marker (NDV).