Figure 2

In vitro and ex vivo transcriptional regulation of chIFIT5 gene. (A) ChIFN-β itself, or produced by different stimuli, can initiate JAK-STAT signalling pathway and culminate in the induction of hundreds of ISGs. IFIT family of genes is one such antiviral effector of innate immune responses. (B) Quantitation of chIFIT5 mRNA in cells stimulated with 1000 U of chIFN-β, 10 μg/mL of LPS, 5 μg/mL of poly I:C or 1 MOI of NDV for 24 hours before RNA extraction and analysis for qRT-PCR using primers specific for the chIFIT5 gene. (C–F) Total cellular RNA was extracted from CEF cells at 1, 2, 4, 8, 16, and 24 hours post-NDV infection and was subjected to quantitation of mRNA for chIFIT5 (C), viral mRNA (D), chMx (E) and chIFN-β (F,G) RNA collected from seven organs infected or not with H9N2 influenza viruses were used to determine the level of chIFIT5 mRNA and M gene of the virus. Fold change induction in all experiments was determined by 2^−∆∆CT algorithm and data presented is average of three independent experiments.