Figure 4

Global methylation inhibitor (DZNep) prevents glucose-induced upregulation of MALAT1, TNF-α, and PRC2 expressions. RT-qPCR findings indicate HG-induced elevations of (A) MALAT1, (B,C) inflammatory transcripts (TNF-α, IL-6), and (D–F) PRC2 components (EZH2, SUZ12, and EED) compared to NG at 48 hours. Such upregulations were prevented (except IL-6) following DZNep treatment. Protein levels of (G) IL-6 and (H) TNF-α showed similar patterns (RNA data expressed as a ratio to β-actin (mean ± SEM); normalized to NG; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, compared to NG or HG; n = 6 from three independent experiments and performed in triplicates; and protein data are expressed as pg/mL).