Figure 3
Alizarin red staining of CLC and HERS cells cultured with or without conditioned medium for 1 week. Massive minerals were found in CLC groups cultured with only EpiCM (A), or IFCM (B) or MFCM (C) while granular minerals were found in HERS cell groups (D: EpiCM; E: IFCM; F: MFCM). CLC cells owned the ability of mineral formation (A) while HERS cells hardly mineralized (D) when cultured without induction. After induction with IFCM and MFCM, both CLC and HERS cells presented significant higher mineral formation than non-induced CLC and HERS cells. MFCM induced the most abundant mineralization in both CLC and HERS cells. Beside, mineralizations in all three CLC cell groups were significantly higher than those in HERS cell groups (G). Scale bars: 100 μm.
