Figure 3 | Scientific Reports

Figure 3

From: A Meloidogyne incognita effector MiISE5 suppresses programmed cell death to promote parasitism in host plant

Figure 3

Spatial and developmental expression of MiISE5. (A) digoxigenin (DIG)-labeled in situ mRNA hybridization. (B) FITC-labeled in situ mRNA hybridization. Both DIG-labeled antisense MiISE5 cDNA probe and 5′ end labeled with FITC cDNA probe localized MiISE5 transcripts within the SVGs. Scale bar = 10 μm. (C) Developmental expression pattern of MiISE5. The relative expression of MiISE5 was quantified using q-PCR in three different M. incognita life stages: pre-J2s, par-J2s (1–3 dpi), and J3s (7–13 dpi). Each bar represents the mean ± SD of three biological replicates; the 18 S gene was used as an internal control. dpi: days postinoculation.

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