Figure 4

MiISE5 can be secreted through M. oryzae infectious hyphae to plant cells. (A) Diagrams of the GFP fusion constructs. The MiISE5-GFP fusion construct pRGTN:MiISE5 was under the control of the RP27 promoter. (B) GFP signals (white arrows) can be observed in the infectious hyphae and plant cells of the M. oryzae transformed with empty vector (pRGTN) and pRGTN:MiISE5, but the GFP signals can only be observed in plant cells penetrated by the infectious hyphae of pRGTN:MiISE5 transformed strain. IH: infectious hyphae. Scale bar = 10 μm.