Figure 1

Examination of cell viability of BV2 cells after low glucose and hypoxia or transfected with vectors and detection of the silencing efficiency of CF in BV2 cells. The external cell morphology appears relatively unchanged in BV2 cells exposed to low glucose and hypoxia for 4 h, 8 h and 12 h when compared with the control (A). Bar graph shows the cell viability of BV2 cells after low glucose and hypoxia compared to control (B). Note that there was no significantly difference in the cells viability of cells in every group (B). The external cell morphology appears relatively unchanged in BV2 cells transfected with control vector or CF shRNA vector when compared with the control (C). Bar graph in the (F) shows the cell viability of BV2 cells transfected with control vector or CF shRNA vector when compared with the control. There was no significantly difference in the cells viability of BV2 cells in every group (F). The silencing efficiency of CF shRNA vector were analyzed by RT-PCR (D), western blots (E). RT-PCR analysis shows that the efficiency of shRNA vector-mediated suppression of CF expression is about 89.27% when compared to control vector cells (D). The upper panel of (E) shows the specific band of CF (20 kDa) and GAPDH (38 kD). Bar graph in the lower panel of (E) shows significant change in the mean optical density of CF protein expression between control vector cells and shRNA vector cells. The protein expression of CF in shRNA vector cells significantly reduced compared with that in control vector cells. *p < 0.05. The values represent the mean ± SEM in triplicate. Scale bars = 100 μm (A and C).