Figure 6
From: Cystatin F involvement in adenosine A2A receptor-mediated neuroinflammation in BV2 microglial cells
qRT-PCR and western blot analysis of CF expression in hypoxic-BV2 cells administrated with H-89, Staurosporine and AG490 before A2A R activation by CGS21680 after 8 h of exposure to low glucose and hypoxia. The bar graph shows significantly changes in the mRNA levels of CF in BV2 cells administrated with H-89, Staurosporine and AG490 before A2A R activation by CGS21680 after 8 h of exposure to low glucose and hypoxia (A). The mRNA levels of CF in H-89 group, Staurosporine group, AG490 group and VEH group which were administrated with CGS21680 significantly increased when compared with control group after 8 h of exposure to low glucose and hypoxia. The mRNA levels of CF in H-89 group and Staurosporine group remarkably decreased when compared with VEH group after 8 h of exposure to low glucose and hypoxia. The (B) shows the specific band of CF dimer (40 kD) and CF monomer (20 kD) in every group. The bar graphs in (C and D) showed significantly changes in the protein expression of CF dimer (C) and CF monomer (D) in BV2 cells administrated with H-89, Staurosporine and AG490 before A2A R activation by CGS21680 after 8 h of exposure to low glucose and hypoxia. The protein expression of CF monomer in H-89 group and Staurosporine group significantly decreased when compared with VEH group after 8 h of exposure to low glucose and hypoxia. There were no significantly differences in the protein expression of CF dimer in every group after 8 h of exposure to low glucose and hypoxia. *p < 0.05. The values represent the mean ± SEM in triplicate.
