Figure 2
Analysis of cross sectional area (CSA) and mean cortical thickness along the entire tibia length. (A) Mean CSA of control and loaded tibiae in female C57/Bl6 at 12 and 22 weeks as well as 20 months of age. (B) Mean cortical thickness of control and loaded tibiae of female C57/Bl6 at 12 and 22 weeks as well as 20 months of age. (C) Representative 3D micro-CT colour-coded images of tibia cortical bone thickness. Statistical significance of differences in CSA and cortical thickness between control and loaded tibiae along the entire tibia shaft, represented as a heat map. Red p < 0.001, yellow 0.001 ≤ p < 0.01, green 0.01 ≤ p < 0.05 and blue p ≥ 0.05. Group sizes were n = 8 for groups1–3 and 4 for group 4 respectively. Group 1: 12 week-old loaded at 11 N; group 2: 22 week-old loaded at 12 N; group 3: 20 month loaded at 9 N and group 4: 20 months old subjected to two episodes of 11 N followed by two weeks of 9 N loading (as in group 3). Line graphs represent means ± SEM.
