Figure 4
From: IL-31 is crucial for induction of pruritus, but not inflammation, in contact hypersensitivity
IL-31 is not essential for skin inflammation during FITC-induced CHS. (a) Wild-type (n = 47) and Il31−/− (n = 41) mice were epicutaneously sensitized and challenged with FITC. The ear skin thickness of the mice was measured at the indicated time points after challenge with FITC or vehicle alone. The data show the mean + SEM. (b) Sections of ear skin from wild-type and Il31−/− mice at 24 hours after challenge with FITC or vehicle alone were stained with hematoxylin and eosin. Representative data are shown. Scale bar = 50 μm. (c) Ninety-six hours after challenge with FITC, sera were collected from wild-type (n = 5 [naïve], and n = 21 [FITC]) and Il31−/− (n = 5 [naïve], and n = 15 [FITC]) mice. The levels of FITC-specific IgG1, IgG2a and IgG2c and total IgE in the sera were determined by ELISA. The data show the mean + SEM.
