Figure 4 | Scientific Reports

Figure 4

From: Iron-specific Signal Separation from within Heavy Metal Stained Biological Samples Using X-Ray Microtomography with Polychromatic Source and Energy-Integrating Detectors

Figure 4

Experimental XRM images of a mouse spinal root, stained with iron and osmium. (a,b) “Low” and “High” energy cross sectional images of spinal root, respectively. Red arrow points to location of node of Ranvier at center of red circle, specifically stained with iron. The circular structures are myelin sheaths stained with osmium. Air and plastic regions were used to calibrate. To ease comparison each image grayscale was set in the range [min, max] = [0, 4x (plastic value)]. In (b), air and plastic regions have been labeled. (c,d) Sagittal and coronal cross sections. Both images (a,b) are cross sections located at position of dashed line labeled slice 1. Images of cross section labeled slice 2 will be discussed in next figure. Enlargements of boxed regions around the marked node (c1-d1) are inserted in each. Scale bars are all 20 μm.

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