Figure 5 | Scientific Reports

Figure 5

From: Iron-specific Signal Separation from within Heavy Metal Stained Biological Samples Using X-Ray Microtomography with Polychromatic Source and Energy-Integrating Detectors

Figure 5

Scatter plots of the spinal root sample. Scatter plots (b,d) were generated by mapping each pixel by its “High” and “Low” attenuation value. Regions within the scatter plot can be traced to specific regions in the slice images shown in (a,c). Slice 1 (a) exemplifies a node in a low staining background. Two large point densities in the scatter plot are associated with air and plastic regions. A node (green circle) and another bright spot (white square) project in the scatter plot at a slope characteristic of iron, as seen when individual pixels associated with ROI are marked similarly in the scatter plot. Background locations with no prominent staining (red diamonds) were taken from the circular dark ring around the node and are associated with plastic. Myelin (black circles) ROI project along a line with a slope characteristic for osmium. Slice 2 (c) exemplifies a node in a region with considerable osmium staining, and so the iron is projected in the scatter plot (d) from a location already exhibiting some given osmium concentration.

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