Figure 6

Scatter plot was used to automatically segment the iron rich nodes of Ranvier regions. Other regions stained with iron were found out to be iron staining of the Schwann cell nucleus. (a) Slice image (b) Scatter plot allowing material analysis using shown material separation lines. (c) Overlay of Material analysis iron image (green) on original slice image. (d) Iron image proportional to iron density, in arbitrary units. All scale bars are 20 μm.