Figure 1
From: Transcriptional Profiling of Somatostatin Interneurons in the Spinal Dorsal Horn
Cell type-specific nuclear tagging and capture of SST neurons in the spinal dorsal horn. (A) SSTGFP mice express the Sun1-GFP nuclear tag in SST neurons in the lumbar dorsal horn (a). The GFP-tagged SST neurons co-localize with the neuronal marker NeuN (red, b), but not with the microglial marker IBA1 (red) or the astrocyte marker GFAP (blue) (c). (d) GFP+ (green) nuclei overlap extensively with native Sst mRNA (red). (B) Schematic of overall experimental workflow. (1) The dorsal segment from one side of the lumbar spinal cord is dissected. (2) Nuclei are liberated by Dounce homogenization in nuclear lysis buffer. (3) Nuclei are then captured using Fluorescence Assisted Nuclear Sorting. (4) The isolated RNA is processed for RNA-seq. (C) GFP+/DAPI+ nuclear were captured from SSTGFP (left). No GFP+ nuclei were present in Sun1-GFP(fl/+) control animals; all DAPI+ nuclei were captured.
