Figure 10
From: DNA Methylation of T1R1 Gene in the Vegetarian Adaptation of Grass Carp Ctenopharyngodon idella
Relative activity of the upstream regulatory region of T1R1 gene. (A) Schematic representation of the upstream regulatory region of T1R1 gene. A series of plasmids containing the upstream sequences of grass carp T1R1 of different lengths (PGL6-T1R1-P, PGL6-T1R1-CPG1−, PGL6-T1R1-CPG3−) fused in the frame to the luciferase were co-transfected with pRL-SV40 into HEK293T cells. (B) Relative activity of the upstream regulatory region of T1R1 gene with deletions. Values were normalized to the control plasmid pGL6-Basic and represented as the ratio between firefly and Renilla luciferase activities. The results are expressed as the mean ± SEM arbitrary units of three independent experiments (P < 0.05).
