Figure 2 | Scientific Reports

Figure 2

From: Effect of the sodium–glucose cotransporter 2 inhibitor luseogliflozin on pancreatic beta cell mass in db/db mice of different ages

Figure 2

Impact of luseogliflozin on beta cell morphology in 6-week-old db/db mice. (a) Histological analysis of pancreatic islets of db/db mice with or without luseogliflozin for 4 weeks. Beta cells are stained brown. (b) Quantitation of beta cell mass in the control group (white bar) and the luseo group (black bar) (n = 8: eight mice were used in each group). (c) Representative insulin (green) and glucagon (red) staining in pancreas sections from the control group and the luseo group. (d) The ratio of the number of beta cells to the sum of alpha plus beta cells in pancreatic islets in the control group (white bar) and luseo group (black bar) (n = 5: five mice were used in each group). (e) Proliferation rate of beta cells assessed using BrdU incorporation in the control group (white bar) and the luseo group (black bar) (n = 8: eight mice were used in each group). (f) Apoptosis rate of beta cells assessed using the TUNEL assay in the control group (white bar) and the luseo group (black bar) (n = 10: ten mice were used in each group). Values are mean ± SD. *p < 0.05; **p < 0.01. Scale bars: 50 μm.

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