Figure 2
From: The stage of seed development influences iron bioavailability in pea (Pisum sativum L.)
Ferritin levels in cotyledons, embryo axis and seed coat. Protein extracts (20 µg) from total pea seeds (JI1194), cotyledons, embryo axis and seed coat at three stages of development were separated by SDS-PAGE for immunoblot analysis (left panels) or by native PAGE for iron staining (right panels). The results are representative of 3 independent biological replicates, and each sample was prepared from 2–3 seeds or tissues.
