Figure 4
From: The stage of seed development influences iron bioavailability in pea (Pisum sativum L.)
Corresponding localization of iron and ferritin protein. Adjacent thin sections of cotyledons from immature pea seeds (JI1194) at 22–24 DAF were labelled with antibodies against pea ferritin followed by Alexa-Fluor conjugated secondary antibodies (top left, green) or imaged for 56Fe16O− ions using NanoSIMS (top right, with colour scale for the intensity of the signal). The differential interference (DI) image of the immunolabelled section and the secondary electron (SE) image for NanoSIMS show the cell walls and organelles. CW, cell wall; Ap, amyloplast. The images are representative of 2 different regions for which 2 cells each were imaged.
