Figure 3 | Scientific Reports

Figure 3

From: Nucleolar-nucleoplasmic shuttling of TARG1 and its control by DNA damage-induced poly-ADP-ribosylation and by nucleolar transcription

Figure 3

EGFP-TARG1 localizes to transcriptionally active nucleoli independent of ADP-ribosylation. (a) Live cell imaging of U2OS cells transiently expressing EGFP-TARG1 or EGFP together with mCherry-H2B. Intensity profiles for EGFP and mCherry fluorescence signals are displayed that were measured along the arrows depicted in the merge pictures. DIC: differential interference contrast. (b) Live cell imaging of U2OS cells transiently expressing EGFP-TARG1 wildtype (WT), G43E, I44E or G123E together with mCherry-H2B. For intensity profiles, see Supplementary Figures S7. (c) U2OS cells transiently expressing EGFP-TARG1 or EGFP-TARG1-G123E together with mCherry-H2B were treated with 10 ng/ml actinomycin D (ActD) or DMSO for 90 min. Subcellular localization of EGFP-TARG1 (wildtype or G123E) was analyzed by confocal microscopy in living cells.

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