Table 1 Summary of the residues important for DNA binding in apo-CjFur and holo-CjFur.

From: Functional insights into the interplay between DNA interaction and metal coordination in ferric uptake regulators

 

Regulatory metal ion

Mutations tested

Key residues

Apo-CjFur EMSA

N.A.

R14E, K17E, R20E, K25E, K28E, R30E, Y68A, R69E

R20, K25, K28, R69

Apo-CjFur RT-qPCR (activation)

N.A.

R20E, K25E, R30E, Y68A, R69E

K25, R30, Y68

Apo-CjFur RT-qPCR (repression)

N.A.

R20E, K25E, R30E, Y68A, R69E

R20, K25, R30, Y68, R69

Holo-CjFur EMSA

Mn

R20E, K25E, K28E, R30E, Y68A, R69E

K25, R30, Y68, R69

Holo-CjFur RT-qPCR (repression)

Fe

R20E, K25E, R30E, Y68A, R69E

R20, K25, R30, Y68, R69

  1. N.A. Not applicable.
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