Figure 5
From: Drosophila Voltage-Gated Calcium Channel α1-Subunits Regulate Cardiac Function in the Aging Heart

Mitochondrial ROS production. ROS generated by isolated mitochondria was measured using amplex red and succinate as a substrate. (A) ROS production by mitochondria isolated from the D-type Ca2+ channel (grey) and wild-type (black) normalized to the protein concentration. Bar graphs represent quantification of A. (B) ROS production by mitochondria isolated from the T-type Ca2+ channel (grey) and wild-type (black) normalized to the protein concentration. Bar graphs represent quantification of B. (C) ROS production by mitochondria isolated from Cac channel (grey) and wild-type (black) normalized to the protein concentration. Bar graphs represent quantification of C.