Figure 6

Cardiac function of Drosophila VGCCs. (A) Representative M-mode OCT traces defined the dimensions of the cardiac tube in a 1-week old wild-type (Canton-S) fly. The end diastolic diameter (EDD, red bar) and end systolic diameter (ESD, blue bar) were the cardiac dimensions used to quantify fractional shortening. The diastolic interval (DI, red arrow) and systolic interval (SI, blue arrow) define the cardiac period (white arrow). (B) Cardiac function was evaluated using M-mode traces of the cardiac tube in wild-type and α1 mutants in relation to age. Enlarged individual events are shown in the red boxes. The prominent arrhythmic events that occurred in the 1 week and 6-week old mutants are shown in the yellow boxes. The cardiac parameters include fractional shortening (C), heart rate (D), end diastolic diameter (E), end-systolic diameter (F) and arrhythmic Index (G). Significant differences between wild-type and mutants were determined by one-way ANOVA followed by a post-hoc Tukey test (*p < 0.05, n = 11 to 16).