Figure 5

Relative barcode frequencies for each lineage over time in different concentrations of amikacin (AMK), cefepime (FEP) and doxycycline (DOX). Each column represents the different conditions: lineages grown in the media without drug (media), media containing sub-inhibitory drug concentrations (0.25 WT IC90) or three different concentrations above the WT IC₉₀ (2×, 4× and 8× WT IC₉₀). The three rows represent the three different drugs. Each plot displays the relative barcode abundance of each lineage over time. Clones adapted to AMK, FEP, DOX and media are displayed in yellow, blue, purple and green, respectively. The intensity of the color increases with the resistance level of each clone towards the drug they were evolved to.