Figure 4 | Scientific Reports

Figure 4

From: Experimental Zika Virus Infection in the Pregnant Common Marmoset Induces Spontaneous Fetal Loss and Neurodevelopmental Abnormalities

Figure 4

In situ hybridization + and − single strand RNA (ssRNA) oligonucleotide probes in spontaneously aborted marmoset (dam 2) placental tissue. The concomitant labeling of both + and − ssRNA is indicative of actively replicating ZIKV. Villous sections were hybridized using amplified probe sets against the +ssRNA the −ssRNA genome to localize active viral replication in the placenta. Fixed Vero cells actively replicating ZIKV at 6 DPI were used as positive controls (A). Placental tissue sections were highly positive for the presence of genomic RNA in villous parenchyma (B,i-ii). Furthermore, ubiquitous detection via ISH of the negative replication strand indicated ZIKV as established in the fetal-placental tissue of the chorionic villi (B,iii-iv). A probe set against human peptidylprolyl isomerase (PPID) mRNA was used as a negative control (B,v-vi). (C) Examination of H&E stained sections of the placental villous revealed no leukocyte infiltration. Dashed boxes denote the zoomed area depicted at high power (60X).

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