Figure 6
From: PrismPlus: a mouse line expressing distinct fluorophores in four different brain cell types
Retention of endogenous fluorophores in PrismPlus mouse brain tissue after CLARITY. (a) Confocal image (snapshot) of a cortex after active CLARITY showing that all fluorophores survived the clearing process. Note that YFP signal is prominent at the nucleolus and the nuclear envelope. Same colored arrow pointing at a particular cell type; Cyan arrow: CFP expressing oligodendrocytes, Green: GFP expressing microglia; Yellow:YFP expressing neurons, and Red: DsRed expressing astrocytes. (b) 3-D render view of 100 microns thick Z- stack of a PrismPlus brain (approximately layer IV-V in cortex) after CLARITY showing CFP expressing oligodendrocytes, GFP expressing microglia and DsRed expressing astrocytes. Figures C,D, and E represent 10 microns thick Z-stack projection at different Z-depths in Fig. B. Due to the punctate YFP, it was excluded in the Figures b,c,d, and e. Image in (b) was taken with a 20x objective with digital zoom of 2.0. Scale bar in a = 100 µm; c = 50 µm.
