Figure 5
SEC-MALS and CIEX-MALS of Adalimumab. (A) SEC-MALS results of Adalimumab. (B) CIEX-MALS results of Adalimumab and a gel image of SDS-PAGE analysis of the eluted peaks in reducing (+DTT) and non-reducing (−DTT) conditions (see Supplementary Fig. S4 for full gel image). The chromatograms display the UV at 280 nm (green), light scattering at 90° angle (red), refractive index (blue) and conductivity (grey) curves together with the molar mass of each peak determined by MALS (black).
