Figure 3
From: FTY720 Decreases Tumorigenesis in Group 3 Medulloblastoma Patient-Derived Xenografts
FTY720 treatment resulted in decreased viability and proliferation of medulloblastoma PDXs. (A) Cell viability was measured using alamarBlue® assays. D425, D341 and D384 cells were treated with increasing concentrations of FTY720 for 24 hours. Viability was significantly decreased in the D341 beginning at a concentration of 4 µM (*p ≤ 0.01). D425 and D384 cells showed significant decreases in viability at concentrations of 6 µM (*p ≤ 0.01). (B) CellTiter 96® assays were used to measure proliferation. D425, D341 and D384 cells were treated with increasing concentrations of FTY720 for 24 hours. Proliferation was significantly decreased in the D341 and D384 cells at 4 µM (*p ≤ 0.01) and at 6 µM (*p ≤ 0.01) in the D425 cells. Experiments were repeated at least in triplicate and reported as mean ± SEM.
