Figure 2 | Scientific Reports

Figure 2

From: The psoriasis-protective TYK2 I684S variant impairs IL-12 stimulated pSTAT4 response in skin-homing CD4+ and CD8+ memory T-cells

Figure 2

Gating strategy for phospho-flow cytometry of p-STAT4. Lymphocytes were gated on a forward (FS) and side scatter (SS) density plot and on a SS area versus SS width density plot to remove doublet cells. A live/dead stain (FVS-450) was used to exclude dead lymphocytes. Live, activated, memory, skin-homing CD4+ or CD8+ T lymphocytes were selected based on the expression of CD25, CD45RO, and cutaneous lymphocyte antigen (CLA). pSTAT4 expression was detected in cells activated by anti-CD3/CD28 and stimulated with or without 50 ng/ml IL-12 for 1 h.

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