Figure 5 | Scientific Reports

Figure 5

From: Ganetespib targets multiple levels of the receptor tyrosine kinase signaling cascade and preferentially inhibits ErbB2-overexpressing breast cancer cells

Figure 5

Ganetespib decreases the protein stability and half-life of ErbB2 in ErbB2+ breast cancer cells. (a) BT474 and SKBR3 cells were treated with CHX (100 μg/mL) +/− ganetespib (1 μM) for the indicated treatment durations. Then, ErbB2 protein expression was analyzed using Western blotting. Cropped images of ErbB2 and β-actin protein expression are shown. (b) ErbB2 protein band intensities were quantified using ImageJ software and normalized to corresponding β-actin protein band intensities. Relative ErbB2 protein expression after CHX +/− ganetespib treatments for each cell line is graphed.

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