Figure 5
Gene expression of HCN4 and Cx40 in NPRA wild type (WT), heterozygous (HET) and knockout (KO) ventricles at E14.5 stage. RT-qPCR analysis was used to monitor HCN4 (A) and Cx40 (B) gene expression levels in the ventricles of indicated NPRA genotypes. Expression levels were normalized to GAPDH via ∆∆CT method. Relative expression levels were presented as fold changes in comparison to the levels in WT. N = 8–13 independent RNA extractions/genotype for both panels, each bar represents mean ± SEM, *P < 0.05 Vs. WT for HCN4 gene expression and *P < 0.05 Vs. WT or HET for Cx40 gene expression. One-way ANOVA with Tukey’s multiple comparisons post hoc test.
