Figure 3 | Scientific Reports

Figure 3

From: Essential role of connective tissue growth factor (CTGF) in transforming growth factor-β1 (TGF-β1)-induced myofibroblast transdifferentiation from Graves’ orbital fibroblasts

Figure 3

TGF-β1, but not TGF-β2 induced up-regulation of fibrosis-related proteins in the GO orbital fibroblasts. (A) After treatment of the GO orbital fibroblasts with 5 ng/ml TFG-β1 and TFG-β2, respectively for 24 hours, the proteins expression of CTGF, fibronectin and α-SMA were examined by Western blots. (B) The ratio of CTGF, fibronectin, and α-SMA expression to each β-actin control from GO1 without TGF-β1 treatment were defined as 1.0, and thus the other relative intensity (folds) were presented, respectively. By three independent Western blots experiments, we averaged together data from the same patients strain (GO1-GO4, N = 4), and then averaged the means of the different patients strain. The representative histogram was constructed on the basis of the mean values of proteins expression levels in orbital fibroblasts of 4 GO patients, and presented as means ± S.D. (*p < 0.05 vs. control without TGF-β1 treatment; **p < 0.01 vs. control without TGF-β1 treatment).

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