Figure 4

CTGF induced the fibronectin and α-SMA proteins expression in GO orbital fibroblasts. (A) After treatment of the orbital fibroblasts from GO1 patient with 50, 100, 200 and 400 ng/ml recombinant human protein CTGF (rhCTGF) for 24 hours, the proteins expression of fibronectin and α-SMA were examined by Western blots. (B) The representative bar graphic was constructed by the data obtained from three independent experiments, and data are presented as means ± S.D. (*p < 0.05; ** and ^##p < 0.01). (C) By an ELISA kit, the intracellular levels of TGF-β1 in the orbital fibroblasts from GO1 patient was examined after rhCTGF treatment for 24 hours. The representative bar graphic was constructed by the data obtained from three independent experiments, and data are presented as means ± S.D. (*p < 0.05 vs. control without rhCTGF treatment; **^,##p < 0.01 vs. control without rhCTGF treatment).