Figure 1
From: Functional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism
(A) The 50-kb nif gene cluster in L. boryana dg5. Genes that were transferred to the genome of Synechocystis 6803 to isolate CN1 are shown in pink. Genes in this central region (shown in two arrows of right and left parts) are divergently transcribed from the nifB (P nifB ) and nifP (P nifP ) promoters (shown by small pink triangles). Expression of all these genes except cnfR is dependent on CnfR29. Genes denoted in yellow show the highest transcript levels under nitrogen fixing conditions and their transcripts are still detected in the absence of cnfR (∆cnfR), albeit lower than wild type29. Subfragments I–V introduced sequentially into the genome neutral site III are shown by red lines. (B) Gene arrangements of the transferred nif gene cluster in Synechocystis 6803 transformants CN1, CN2, and CN3. Subfragments VI, VII, and VIII (red lines) were used for isolation of CN1, CN2, and CN3, respectively.
