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Figure 2

From: The orphan germinant receptor protein GerXAO (but not GerX3b) is essential for L-alanine induced germination in Clostridium botulinum Group II

Figure 2

Relatedness of GerX3b receptor gene clusters in Clostridium botulinum Group II and genetic organization of the gerX subtypes. (a) Relatedness of gerX3b types in C. botulinum Group II (toxin types shown in coloured boxes). gerX associated proteins used for comparisons; gerX3bA (Locus tag; CB17B_RS15165), gerX3bB (Locus tag; CB17B_RS15170), gerX3bC (Locus tag; CB17B_RS15160) and gerXAO (Locus tag; CB17B_RS12225). *Represents strain C. botulinum Eklund 17B (NRP) (Accession number; FR745875) used in this study. †Represents strain C. botulinum NCTC 11219 for comparison. Clade I consist of strains forming neurotoxin type E, and Clade II includes strains forming type neurotoxin types B, E or F. Strains used for comparison of the gerX3b types see Brunt et al.37. The phylogenetic tree is based on single nucleotide polymorphisms (SNPs). Values shown at branches represent bootstrap values provided by ParSNP. (b) Genetic organization of the gerX3b gene cluster. (c) gerXAO is flanked by asparagine synthetase (asnB) and an uncharacterized S1 RNA binding domain protein (yitL).

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