Figure 3 | Scientific Reports

Figure 3

From: The orphan germinant receptor protein GerXAO (but not GerX3b) is essential for L-alanine induced germination in Clostridium botulinum Group II

Figure 3

Confirmation of insertional mutagenesis. (a) PCR screens of C. botulinum gerX gene mutants confirming that the intron has successfully inserted into the target gene. Numbers represent primer sets used. Gel image has been cropped, full image available in supplementary figure (S1). (b) C. botulinum germination genes showing Clostron insertion sites (red arrows). Black arrows represent primer annealing sites. Primer set 1 (gerC-F/R) anneals to the target gene CB17B_RS15160 gerX3bC either side of the intron insertion site and confirms the intron is present in the target gene (expected band size of mutants ~2 kb). Primer set 2 (gerAB-F/R) anneals to the target gene CB17B_RS15165 gerX3bA and CB17B_RS15170 gerX3bB either side of the intron insertion sites and confirms the intron is present in the target gene. Primer set 3 (gerAO-F3/R3) anneals to the target gene CB17B_RS12225 gerXOA either side of the intron insertion sites and confirms the intron is present in the target gene.

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