Figure 4 | Scientific Reports

Figure 4

From: The orphan germinant receptor protein GerXAO (but not GerX3b) is essential for L-alanine induced germination in Clostridium botulinum Group II

Figure 4

Effect of L-alanine (50 mM) on spore Germination of C. botulinum Eklund 17B gerX3bA−, gerX3bB−, gerX3bC−, gerXAO− and wild type. (a) Kinetics of spore germination determined using fall in OD600. (b) Spore germination after 3 hours (percentage of phase dark spores as determined by phase contrast microscopy). (c) Spore germination after 24 hours (percentage of phase dark spores as determined by phase contrast microscopy). (d) Kinetics of spore germination of the complemented mutants determined using fall in OD600. Error bars represent the standard deviation of three independent experiments. *Student’s T-test with a significance level of 0.05. **Not significant at level of 0.05 compared to the wild type. +complemented strains.

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