Table 6 List of PCR primer pairs used for the real-time PCR analysis.

From: Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus

Primers

Forward (5′-3′)

Reverse (5′-3′)

SOCS3

TTTCTTCACCCTGTCCGTGC

CCAGCCCTTTCCCCATGTAG

JNK1

TGGTCCGGGGTAGTGTGTTG

TCTCTGGCTTGGCTCGCTTT

PI3K

GCTCATCAACCACTATCGC

TGTCTTCTTTCACCACCTG

IRS1

CCCACTTAGGAAAAGCAGAG

AGTACAGGAACGGAAGGATC

IRS2

GGAGGTATGGCAAGTGAAT

AAGAAGAAGCTGTCGGAGT

AKT1

GAGGGAAGAATGGACGAAAG

TTCCCAGGAGTTTGAGGTAT

AKT2

CATCCCTTTCTAACAACACTA

CTGTAAACAACATTGCGTGA

GLUT2

GAACAGCACAGAAGAAGAGG

ACAGCCAGAACATTGACCAT

PK

GCTSGACTACAAGAACATC

CTCGTGGTTCTCCAGYTTG

GK

GGGATGATTGTTGGCACT

TGGAACCTGTCACGGAAA

PFK

TTGTAATCGGAGGGTTCG

ATTGTTGCTGATGGTGGC

β-ACTIN

GGAAATCGTGCGTGACATTAAG

CCTCTGGACAACGGAACCTCT

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